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[IRX60 in Agroforestry Science] Customer article: Dual inhibition and defense induction of citrus green mold by Sanxia peptidin revealed

Release time:Release time:2024 - 08 - 09

Citrus is one of the most popular fruits in the world due to its high nutritional value and various health-promoting properties. However, after harvest, citrus is susceptible to fungal infections during storage, transportation, and marketing, leading to significant economic losses and potential food safety crises.


Penicillin green mold is the main cause of citrus rot, accounting for about 90% of rotting citrus. Currently, the control of citrus storage rot mainly relies on chemical fungicides, but the widespread use of these chemicals may lead to fungal resistance as well as environmental and health concerns. Therefore, a natural antifungal alternative is urgently needed.


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Recently, Prof. Shiping Liu's team from Three Gorges University published their research results entitled “Sanxiapeptin is an ideal preservative with a dual effect of controlling green mold and inducing systemic defense in postharvest citrus” (IF=8.8) in Food Chemistry, the top international agri-food journal. mold and inducing systemic defense in postharvest citrus” (IF=8.8), which verified that Sanxiapeptin can inhibit penicillin green mold through a multiphase antifungal mechanism and effectively control the storage rot of citrus.

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The research team extracted and purified Sanxiapeptin from Penicillium spp. by high-performance liquid chromatography (HPLC), and assessed the effects of Sanxiapeptin on spore germination, germ tube length, and mycelial growth through multiple concentration gradient experiments.



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▲ Fluorescence analysis of 0 (A1, B1, C1), 30 (A2, B2, C2), and 60 μg/mL (A3, B3, C3) Sanxiapeptin-treated P. digitatum


Among them, higher green fluorescence intensity indicates more severe cell membrane damage, and higher blue fluorescence intensity indicates greater changes in cell wall structure. Meanwhile, the researchers quantitatively analyzed the fluorescence intensity in SOPTOP image analysis software to assess the degree of cell membrane and cell wall damage.



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In this study, by integrating in vitro and in vivo findings, we elucidated the multiple antifungal mechanisms of action of Sanxiapeptin (Sanxiapeptin) and demonstrated the great potential of Sanxiapeptin for the control of citrus green molds, which has the prospect of application as a novel food preservative.


Paper Information

Li J, Bai X, Zhu G, Liu S, Liu C, Wu M, Zou K, Li A, Liu S. Sanxiapeptin is an ideal preservative with a dual effect of controlling green mold and inducing systemic defense in postharvest citrus. Food Chem. 2024 Sep 30;453:139669. doi: 10.1016/j.foodchem.2024.139669. Epub 2024 May 14. PMID: 38781900.

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